N,N-Dimethyltryptamine
DMT Mass Spectra
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DMT Mass Spectra (expanded)
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Quantification
- Blood:
- GC Column: DB-1 fused silica capillary (30 m  0.32 mm i.d., 0.2 mm). Carrier gas: H2, 3 or 3.5 mL/min. Temperature: 230° and 280° . SID. Limit of
detection, 0.5 mg/L [Ishii et al. 1997].
- GC-MS Column: SE-30 (18 m  0.33 mm i.d.). Carrier gas: He, 2 mL/min. Temperature: 200° . EI ionisation at 70 eV, SIM acquisition mode. Limit of detection, 10 ng/L [Walker et al. 1979].
- Plasma GC Column: 5% phenyl methyl silicone capillary (12 m  0.2 mm i.d.,0.33 mm). Carrier gas: H2, 0.7 mL/min. Temperature programme: 70° for 1 min to 120° at 30° /min to 280° at 20° /min. Limit of quantification, 1.6 mg/L [Yritia et al. 2002].
- HPLC Column: Supelcosil LC-DB-8 (150 Â 4.6 mm i.d., 5 mm). Mobile phase: methanol : acetonitrile : 0.1 mol/L ammonium acetate (pH 6.9, 20 : 20 : 60), flow rate 2.0 mL/min. Fluorescence detection (lex1⁄4 232 nm, lem1⁄4 351 nm or lex1⁄4 340nm, lem1⁄4 495 nm). Limit of quantification, 2 mg/L [Callaway et al. 1996].
- Urine
- GC See Blood [Ishii et al. 1997].
- GC-MS Column: 1% OV-101 on 80/100 mesh Gas-Chrom Q. Temperature:190° . EI ionisation, MID. Limit of detection, 100 ng/L [Raisanen, Karkkainen 1979].
- LC-MS EI ionisation at 70 eV. SRM acquisition mode. Limit of detection, 2–10 mg/L [Bjornstad et al. 2009]. Column: Brownlee O Spheri-5 RP-18 (100 Â 1.0 mm i.d., 5 mm). Mobile phase: methanol : water (50 : 50) with 0.2% formic acid, flow rate 40 mL/min.
- ESI, MRM acquisition mode. Limit of detection, 0.1 mg/L [Forsstrom et al. 2001].
IR - Infrared
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Other IR data (Clarke's second): Principal peaks at wavenumbers 743, 1113, 1235, 1050, 812, 1010 (KBr disk)
UV-Vis
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Other UV-Vis data:
- λmax 222nm (log e 4.48), 277 (3.77) and 288 (3.75) Ghosal et al 1969
- λmax 222-224, 274 & 294nm Banergee & Ghosal 1969
- λmax 222, 277, 287 & 294 nm Ghosal & Banergee 1969
- λmax 274, 283, 291nm (refernce material) 275, 283, 291nm (isolated material) Fish et al 1955
- λmax (CH3OH): 220, 280, 290 (= 5500, 5600, 5000) De Moraes et al 1990
- λmax (EtOH): 226, 275 (sh), 279, 284, 293nm Grina et al 1982
- λmax 276, 282, 290nm
λmin 278, 287nm Martin & Alexander 1968
- λmax 275, 219 (0.1N NaOH)
- λmax 290, 276, 282 (EtOH) Sunshine 1981
- λmax of Xanthydrol reactive product (CHCl3): 510nm
λmin of Xanthydrol reactive product (CHCl3): 400nm Gander et al 1976
- Quantification. Average between: Ab@238/737*10000 = X ug/ml
Ab@308/296.1*10000 = X ug/ml
NMR
(NMR info source, method description and results discussion:Microgram bulletin volume 5, n14, pg6 )
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Scientific publications
Links of interest
