DR ANTHONY MELVIN CRASTO,WorldDrugTracker, helping millions, A 90 % paralysed man in action for you, I am suffering from transverse mylitis and bound to a wheel chair, With death on the horizon, nothing will not stop me except God................DR ANTHONY MELVIN CRASTO Ph.D ( ICT, Mumbai) , INDIA 25Yrs Exp. in the feld of Organic Chemistry,Working for GLENMARK GENERICS at Navi Mumbai, INDIA. Serving chemists around the world. Helping them with websites on Chemistry.Million hits on google, world acclamation from industry, academia, drug authorities for websites, blogs and educational contribution

Thursday, 16 July 2015

Fragmentation in mass spectrometry

Fragmentation


When molecules go through a mass spectrometer, some of them arrive intact at the detector, but many of them break into pieces in a variety of different ways. To establish a charge on a molecule, an electron had to be removed; removal of that electron is effected through a collision, usually with a high-energy electron. During that collision, energy is transferred from the high-energy electron to the molecule, and that energy has to go somewhere. Part of it gets partitioned into various bond vibrations, so bonds start to vibrate quite a lot, until some of them snap completely. The molecular ion breaks apart and forms a fragment ion.
Some fragment ions are very common in mass spectrometry. These ions are seen frequently for either of two reasons:
  • there is not a pathway available to break the ion down.
  • the ion is relatively stable, so it forms easily.
Fragmentations occur through well-defined pathways or mechanisms. A mechanism is a step-by-step series of events that happens in a reaction. It is important to understand how reactions happen, but we will look at fragmentations when we study radical reactions.
However, it is useful to know what factors make cations stable.

Some Common Ions
There are a number of ions commonly seen in mass spectrometry that tell you a little bit about the structure. Just like with anions, there are a couple of common factors influence cation stability:
  • Electronegativity plays a role. More electronegative atoms are less likely to be cations.
  • Polarizability also plays a role. More polarizable atoms are more likely to be cations.
However, in most cases, we will be looking at carbon with a positive charge, and there are additional factors to distinguish between them
  • Delocalization stabilizes a cation by spreading out the charge onto two or more different atoms.
  • In Lewis structure terms, the easiest way to delocalize charge is via resonance.
  • Resonance can involve other carbons, like in allyl and benzyl cations.
  • Resonance can also involve other atoms, like in acylium or iminium cations.


  • Delocalization can also be accomplished through inductive effects. The trend in carbocations is that the more substituents on teh carbocation, the greater the stability.
  • Tertiary cations, with three substituents on the carbocation, are more stable than secondary cations, with two substituents on the carbocation. Secondary cations are more stable than primary ones. Primary cations are more stable than methyl cations.
Molecular orbital calculations suggest that the cation is stabilized through interaction with neighboring C-H bonds in the alkyl groups. Specifically, a C-H sigma bonding orbital has symmetry similar to the empty p orbital on the positive carbon. The lobes on the two orbitals can overlap such that they are in phase, and that allows electrons to be donated from the C-H bond to the central, electron-deficient carbon. Formally, there is a bonding interaction and an antibonding interaction between these two orbitals. Since one of these orbitals is empty, the antibonding combination remains unoccupied. The bonding combination is populated, however, and since it is lower in energy than either the p orbital or the C-H sigma bond (all bonding combinations are lower in energy than the orbitals that combine to form them), there is a net decrease in energy.



 Fragmentation Patterns
The fragmentation of molecular ions into an assortment of fragment ions is a mixed blessing. The nature of the fragments often provides a clue to the molecular structure, but if the molecular ion has a lifetime of less than a few microseconds it will not survive long enough to be observed. Without a molecular ion peak as a reference, the difficulty of interpreting a mass spectrum increases markedly. Fortunately, most organic compounds give mass spectra that include a molecular ion, and those that do not often respond successfully to the use of milder ionization conditions. Among simple organic compounds, the most stable molecular ions are those from aromatic rings, other conjugated pi-electron systems and cycloalkanes. Alcohols, ethers and highly branched alkanes generally show the greatest tendency toward fragmentation.

The mass spectrum of dodecane on the right illustrates the behavior of an unbranched alkane. Since there are no heteroatoms in this molecule, there are no non-bonding valence shell electrons. Consequently, the radical cation character of the molecular ion (m/z = 170) is delocalized over all the covalent bonds. Fragmentation of C-C bonds occurs because they are usually weaker than C-H bonds, and this produces a mixture of alkyl radicals and alkyl carbocations. The positive charge commonly resides on the smaller fragment, so we see a homologous series of hexyl (m/z = 85), pentyl (m/z = 71), butyl (m/z = 57), propyl (m/z = 43), ethyl (m/z = 29) and methyl (m/z = 15) cations. These are accompanied by a set of corresponding alkenyl carbocations (e.g. m/z = 55, 41 &27) formed by loss of 2 H. All of the significant fragment ions in this spectrum are even-electron ions. In most alkane spectra the propyl and butyl ions are the most abundant.
The presence of a functional group, particularly one having a heteroatom Y with non-bonding valence electrons (Y = N, O, S, X etc.), can dramatically alter the fragmentation pattern of a compound. This influence is thought to occur because of a "localization" of the radical cation component of the molecular ion on the heteroatom. After all, it is easier to remove (ionize) a non-bonding electron than one that is part of a covalent bond. By localizing the reactive moiety, certain fragmentation processes will be favored. These are summarized in the following diagram, where the green shaded box at the top displays examples of such "localized" molecular ions. The first two fragmentation paths lead to even-electron ions, and the elimination (path #3) gives an odd-electron ion. Note the use of different curved arrows to show single electron shifts compared with electron pair shifts.
The charge distributions shown above are common, but for each cleavage process the charge may sometimes be carried by the other (neutral) species, and both fragment ions are observed. Of the three cleavage reactions described here, the alpha-cleavage is generally favored for nitrogen, oxygen and sulfur compounds. Indeed, in the previously displayed spectra of 4-methyl-3-pentene-2-one and N,N-diethylmethylamine the major fragment ions come from alpha-cleavages. Further examples of functional group influence on fragmentation are provided by a selection of compounds that may be examined by clicking the left button below. Useful tables of common fragment ions and neutral species may be viewed by clicking the right button.
 
 
The complexity of fragmentation patterns has led to mass spectra being used as "fingerprints" for identifying compounds. Environmental pollutants, pesticide residues on food, and controlled substance identification are but a few examples of this application. Extremely small samples of an unknown substance (a microgram or less) are sufficient for such analysis. The following mass spectrum of cocaine demonstrates how a forensic laboratory might determine the nature of an unknown street drug. Even though extensive fragmentation has occurred, many of the more abundant ions (identified by magenta numbers) can be rationalized by the three mechanisms shown above. Plausible assignments may be seen by clicking on the spectrum, and it should be noted that all are even-electron ions. The m/z = 42 ion might be any or all of the following: C3H6, C2H2O or C2H4N. A precise assignment could be made from a high-resolution m/z value (next section).

Odd-electron fragment ions are often formed by characteristic rearrangements in which stable neutral fragments are lost. Mechanisms for some of these rearrangements have been identified by following the course of isotopically labeled molecular ions.




No comments:

Post a comment