Resistance to Nucleotide Excision Repair of Bulky Guanine Adducts Opposite Abasic Sites in DNA Duplexes and Relationships between Structure and Function

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Fig 1. Chemical structures of the lesions and sequences.

(A) Chemical structures of the trans-B[a]P-dG, cis-B[a]P-dG and the THF site. The THF is a stable analog of an abasic (AB) site. (B) The sequences and numbering system of the 11-mer duplexes containing the trans-B[a]P-dG adduct. G* denotes B[a]P-dG adduct.

doi:10.1371/journal.pone.0137124.g001

Citation: Liu Z, Ding S, Kropachev K, Lei J, Amin S, Broyde S, et al. (2015) Resistance to Nucleotide Excision Repair of Bulky Guanine Adducts Opposite Abasic Sites in DNA Duplexes and Relationships between Structure and Function. PLoS ONE 10(9): e0137124. doi:10.1371/journal.pone.0137124

Fig 2. 1D and 2D NMR spectra characteristics of the trans-B[a]P-dG:AB duplex.

(A) 1D spectrum (10.5–14 ppm) showing the imino proton assignments. (B) Portion of a 2D NOESY (250 ms mixing time) contour plot recorded at 10°C, in 10% H2O solution showing NOE connectivities between amino (5–8.5 ppm) and imino protons (10.8–13.8 ppm), and (C) imino- imino protons (10.8–13.8 ppm). Assignments: a, T20(NH3)—G21(NH1); b, T14(NH3)—G13(NH1); c, T4(NH3)—G18(NH1); d, T8(NH3)—G16(NH1); e, A3(H2)—T20(NH3); f, A9(H2)—T14(NH3); g, A19(H2)—T4(NH3); h, A15(H2)—T8(NH3); i,i’, C2(NH,H’)—G21(NH1); j, C2(H5)—G21(NH1); k, k’, C10(NH,H’)—G13(NH1); l, C10(H5)—G13(NH1); m, m’, C5(NH,H’)—G18(NH1); n, C5(H5)—G18(NH1); o, o’, C7(NH,H’)—G16(NH1); p, C7(H5)—G(NH1); q, A3(H2)—G21(NH1); r, A9(H2)—G13(NH1); s, A19(H2)—G18(NH1); t, A15(H2)—G16(NH1).

doi:10.1371/journal.pone.0137124.g002......http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0137124