Four antibacterial monoterpenoid derivatives from the herba of Senecio Cannabifolius less

The plant material was dipped into water overnight (1:10) and then extracted for three times after the material had been boiled for 2 h. Filtration and rotary evaporation of the resulting extract gave a residue (780 g), which was dissolved in water and extracted with chloroform, ethyl acetate, and n-butanol. The n-butanol layer was passed through a lowbar ODS column (1.873 g) eluted with MeOH-H ₂ O (5:95, 1:9 v/v) and by HPLC with 15%MeOH-H ₂ O-1%THF to furnish 1 (31.5 mg, [Figure - 1], 2 (16.3 mg,[Figure - 2]), 3 (11.6 mg, [Figure - 3]), and 4 (11.5 mg, [Figure - 4]).

Compound 1:Elution of the column with MeOH-H ₂ O (5:95) afforded yellow liquid 1. IR umax (KBr): 3396, 2928, 1710, 1669, 1365, 1242, 1157, 1077 cm ^-1sub . HRESIMS (positive) m/z : 403.1959[M+H]+ (calcd. for C ₁₉ H ₃₁ O ₉ , 403.1963). ¹sub H-NMR (400 MHz, DMSO- d ₆ ): d5.76 (1H, s, H-1), 2.11 (3H, s, CH ₃ -4), 2.25 (1H, dd, J=2.0; 12.8 Hz, H ₂ -3'a), 1.26 (1H, m, H ₂ -3'b), 4.16 (1H, m, H-4'), 1.97 (1H, dd, J=2.4; 12.4 Hz, H ₂ -5'a), 1.35 (1H, m, H ₂ -5'b), 1.28 (3H, s, CH ₃ -7'), 1.31 (3H, s, CH ₃ -8'), 1.07 (3H, s, CH ₃ -9'), 5.09 (1H, s, OH-2'), 4.24 (1H, d, J=8.0 Hz, H-1''), 2.89 (1H, dt, J=8.0; 4.4 Hz, H-2''), 3.07 (1H, m, H-3''), 3.13 (1H, m, H-4''), 3.10 (1H, m, H-5''), 3.66 (1H, ddd, J=2.0; 5.2; 10.4 Hz, H ₂ -6''a), 3.45 (1H, ddd, J=5.2; 5.2; 10.4 Hz, H ₂ -6''b), 4.88 (1H, d, J=4.4 Hz, OH-2''), 4.88 (1H, m, OH-3''), 4.91 (1H, m, OH-4''), 4.46 (1H, t, J=5.2 Hz, OH-6'').¹³sub C-NMR (100 MHz, DMSO- d ₆ ): d99.6 (C-1), 208.9 (C-2), 197.6 (C-3), 26.2 (C-4), 118.4 (C-1'), 70.4 (C-2'), 46.0 (C-3'), 70.6 (C-4'), 46.9 (C-5'), 35.5 (C-6'), 30.3 (C-7'), 28.7 (C-8'), 31.6 (C-9'), 101.2 (C-1''), 73.4 (C-2''), 76.7 (C-3''), 70.0 (C-4''), 76.7 (C-5''), 61.0 (C-6'').

http://www.ijpsonline.com/article.asp?issn=0250-474X;year=2006;volume=68;issue=3;spage=332;epage=336;aulast=Wu RESEARCH PAPER

Year : 2006  |  Volume : 68  |  Issue : 3  |  Page : 332-336

Four antibacterial monoterpenoid derivatives from the herba of Senecio Cannabifolius less

B Wu1, WH Lin2, HY Gao1, L Zheng1, LJ Wu1, CS Kim3 1 School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China-110 016, 2 School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China-110 016, 3 Faculty of Agriculture, Kochi University, Nankoku, Japan-783-8502,  Correspondence Address: L J Wu School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China-110 016  TAKE A TOUR ರಾಯಚೂರು RAICHUR, Karnataka, India Raichur City in India Raichur is a city municipality in the district of Raichur in the south indian state of Karnataka. Raichur, located between Krishna and Tungabhadra rivers, is the headquarters of Raichur district. Wikipedia Historical Stone Elephants in Malayabad, Raichur Taluk ... View of Raichur city and lake Aam Talab

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